## Dissertations from ProQuest

#### Title

Molecular cloning of rat liver glycogen synthase

1990

Article

#### Degree Name

Doctor of Philosophy (Ph.D.)

#### Department

Biochemistry and Molecular Biology

#### First Committee Member

Ernest Y. C. Lee, Committee Chair

#### Abstract

The cDNA for rat liver glycogen synthase was isolated by screening a rat liver cDNA library constructed in $\lambda$gt11. The cDNA was 2.4 kb in length and encoded a protein of 703 amino acid residues with a molecular mass of 80.5 kDa. Comparison of the rat liver and the human muscle sequences show that the N-terminal and C-terminal regions are quite divergent as compared to the internal sequences which show an 80% identity. The rat liver C-terminal region is truncated by 33 residues and has only 46% identity with the muscle sequence but retains the common feature of a low content of hydrophobic amino acids (13%). Phosphorylation sites 1a and 1b, which are the primary targets for phosphorylation by cAMP-dependent protein kinase, are absent in the liver sequence. The presence of these divergent, structurally anomalous C-terminal regions in liver and muscle glycogen synthase suggests the absence of the requirement that they possess a tertiary structure that is integral to that of the protein core. A model is proposed in which this region interacts with a catalytic core to maintain the I state, and in which phosphorylation serves to uncouple this interaction.

#### Keywords

Biology, Molecular

#### Link to Full Text

http://access.library.miami.edu/login?url=http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:9114788

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