Title

Identification, characterization, and partialcDNA sequence analysis of a novel vesicle-associated protein (VAP-1) from the sea urchin Strongylocentrotus purpuratus

Date of Award

1990

Availability

Article

Degree Name

Doctor of Philosophy (Ph.D.)

Department

Cellular and Molecular Biology

First Committee Member

Melanie M. Pratt, Committee Chair

Abstract

A high molecular mass (approx. 600 kDa), vesicle-associated protein (VAP-1) has been identified in the eggs of the sea urchin S. purpuratus. Characterization of this protein reveals that it is peripherally associated with a population of acidic vesicles in the egg. Additionally, VAP-1 is phosphorylated on serine and/or threonine residues and it is sensitive to calcium-dependent proteolytic modification. Partial cDNA sequence analysis suggests that VAP-1 may function as an RNA-binding protein. The potential functions of VAP-1 during early sea urchin development are discussed, including possible roles as a cytoplasmic RNA-binding protein involved in mRNA localization or as a nuclear protein precursor that will be translocated to specific nuclei later in development to function in RNA processing or splicing.

Keywords

Biology, Molecular; Biology, Cell; Chemistry, Biochemistry

Link to Full Text

http://access.library.miami.edu/login?url=http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:9114789