Title

Molecular recognition at the interface: A Langmuir and Langmuir-Blodgett film study

Date of Award

1999

Availability

Article

Degree Name

Doctor of Philosophy (Ph.D.)

Department

Chemistry

First Committee Member

Roger M. Leblanc, Committee Chair

Abstract

Molecular recognition plays an essential role in many functions of membrane surfaces. Real time detection of molecular recognition processes is an important step for the investigation of biological phenomena as well as for biosensor development. Langmuir monolayers and Langmuir-Blodgett (LB) films are suitable membrane mimic systems. The Langmuir monolayers and LB films of two different molecular systems were studied in this thesis. They are diacetylene lipid derivatives and fullerene derivatives. The interfacial, spectroscopic and topographic properties of these systems under different conditions were investigated. Interactions of the monolayers and/or LB films of these molecules with ions and/or ligands at the air-liquid and/or solid-liquid interfaces were monitored and analyzed.The diacetylene derivatives with sugar headgroup can form miscible monolayers with diacetylenic acid. The molecular recognition of these mixed monolayers to protein Concanavlin A was studied by surface chemistry and fluorescent labeling techniques. The photo-induced polymeric and chromatic properties of different diacetylene derivatives were studied. A chemical and photochemical induced dual polymerization was found for the first time in the mixed monolayer of siloxane and diacetylene derivatives. The mixed monolayers of diacetylene acid with semi-fluorinated alkane show unusual interfacial properties. The monolayers of different dipeptide-diacetylene derivatives prove that the headgroup size has a remarkable influence on the chromatic properties of the polydiacetylene.The monolayers of fullerene derivatives with different size of crown-ether hydrophilic group can be stabilized upon binding to the subphase cations. The Langmuir monolayer and LB films of these molecules show different spectroscopic properties upon photo-irradiation. The C60 derivatives containing carbohydrate dendrimers are the first fullerene derivatives that can form very stable Langmuir monolayer and LB films.

Keywords

Chemistry, Analytical; Chemistry, Physical

Link to Full Text

http://access.library.miami.edu/login?url=http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:9948792