Doctor of Philosophy (PHD)
Biochemistry and Molecular Biology (Medicine)
Date of Defense
First Committee Member
Arun Malhotra - Committee Chair
Second Committee Member
Thomas K. Harris - Committee Member
Third Committee Member
Miguel Perez-Pinzon - Committee Member
Fourth Committee Member
Antoni Barrientos - Mentor
Fifth Committee Member
Carla Koehler - Outside Committee Member
Copper is an essential cofactor of two mitochondrial enzymes: cytochrome c oxidase (COX) and the mitochondrial localized fraction of Cu-Zn superoxide dismutase (Sod1p). Copper incorporation into these enzymes is facilitated by a growing number of metallochaperone proteins. Here we describe two novel copper chaperones of COX, Cmc1 and Cmc2. In Saccharomyces cerevisiae, both Cmc1 and Cmc2 localize to the mitochondrial inner membrane facing the intermembrane space. Cmc1 and Cmc2 are essential for full expression of COX and cellular respiration, contain a twin Cx9C domain, and are conserved from yeast to humans. Additionally, the presence or absence of these proteins not only determines full assembly of functional COX but also affects metallation of Sod1 suggesting these proteins might play a role on co-modulation of copper transfer to COX and Sod1. CMC1 overexpression does not rescue the respiratory defect of cmc2 mutants or vise versa. However, Cmc2 physically interacts with Cmc1 and the absence of Cmc2 induces a 5-fold increase in Cmc1 accumulation in the mitochondrial membranes. We conclude that Cmc1 and Cmc2 have cooperative but non-overlapping functions in cytochrome c oxidase biogenesis.
Copper; Cmc1; Cmc2 Mitochondria; Cytochrome C Oxidase; Superoxide Dismutase
Horn, Darryl M., "Characterization of Two CX9C Containing Mitochondrial Proteins Necessary for Cytochrome c Oxidase Assembly" (2010). Open Access Dissertations. 375.