Larval rearing and settlement of the green mussel, Perna viridis

Date of Award




Degree Name

Doctor of Philosophy (Ph.D.)


Biology and Living Resources

First Committee Member

Larry E. Brand - Committee Chair

Second Committee Member

Edwin S. Iversen - Committee Member


Larvae of the green mussel (Perna viridis) were reared in the laboratory to determine optimum conditions for growth, survival and settlement. In the test for the most suitable diet with sixteen algal species, Isochrysis aff galbana (TI) gave the best larval growth although it was not significantly different from Skeletonema costatum, Pavlova lutheri, Emiliania huxleyi and Isochrysis galbana (Iso). From the biochemical analyses of the algal diets and correlation with larval growth, a significant positive correlation between algal lipid content and growth of the mussel larvae was obtained. Feeding with single and mixed algal diets for three weeks resulted in significant differences in survival of the mussel larvae but no significant difference was observed among larval growth rates.In establishing the optimum feeding level for the mussel larvae, maximum shell length was obtained at a ration of 2,500 cells/larva/day of Isochrysis aff galbana (TI) for 70 and 82-um larvae and at a ration of 5,000 cells/larva/day for 190-um larvae. Rearing the mussel larvae at 5 larvae/ml produced the highest growth and survival and at 50 larvae/ml, the lowest growth and survival were obtained.Of the ten substrate materials tested for suitability to larval settlement, cotton had the highest number of settling larvae although this was not significantly different from the other substrates such as acrylic yarn, nylon, plastic screen, sisal and jute. Substrate materials with rough surfaces had higher larval settlement compared to those with smooth surfaces. In an attempt to enhance larval settlement, $\gamma$-amino-n-butyric acid was added to the larval cultures, but this did not result in a significant increase in larval settlement compared with the control treatment.


Biology, Oceanography; Agriculture, Fisheries and Aquaculture

Link to Full Text


Link to Full Text