Title

Identification, characterization, and partialcDNA sequence analysis of a novel vesicle-associated protein (VAP-1) from the sea urchin Strongylocentrotus purpuratus

Date of Award

1990

Availability

Article

Degree Name

Doctor of Philosophy (Ph.D.)

Department

Cellular and Molecular Biology

First Committee Member

Melanie M. Pratt - Committee Chair

Abstract

A high molecular mass (approx. 600 kDa), vesicle-associated protein (VAP-1) has been identified in the eggs of the sea urchin S. purpuratus. Characterization of this protein reveals that it is peripherally associated with a population of acidic vesicles in the egg. Additionally, VAP-1 is phosphorylated on serine and/or threonine residues and it is sensitive to calcium-dependent proteolytic modification. Partial cDNA sequence analysis suggests that VAP-1 may function as an RNA-binding protein. The potential functions of VAP-1 during early sea urchin development are discussed, including possible roles as a cytoplasmic RNA-binding protein involved in mRNA localization or as a nuclear protein precursor that will be translocated to specific nuclei later in development to function in RNA processing or splicing.

Keywords

Biology, Molecular; Biology, Cell; Chemistry, Biochemistry

Link to Full Text

http://access.library.miami.edu/login?url=http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:9114789

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