Doctor of Philosophy (PHD)
Biology (Arts and Sciences)
Date of Defense
First Committee Member
Carol C. Horvitz - Committee Chair
Second Committee Member
David P. Janos - Committee Member
Third Committee Member
Leonel da S. L. Sternberg - Committee Member
Fourth Committee Member
Josiane Le Corff - Outside Committee Member
Monoecy, the production of distinct male and female flowers on the same plant, is an important, though little studied, sexual strategy in the rainforest understory. This study of a monoecious plant discovered a cue to induce flowering, explored the interplay of gender constraint vs. plasticity in a natural population, and tested possible causes of gender in two laboratory experiments. An experiment in the lab found that reduced photoperiod for three weeks is an unambiguous cue for flowering. The remarkably long inductive period is followed by a long and variable period of floral initiation. This results in only partial synchronization of flowering among plants in a patch, which enhances mating opportunities in this protandrous plant. Inflorescence architecture is highly constrained, and ideally produces a phenotypic gender (proportion female) of about 0.5. However, in the forest at Las Cruces, Costa Rica, most plants were less female than predicted, mostly through abortion of female buds. Plants showed gender plasticity between and within years. Large plants produced more flowers and were more female in gender, and less variable in gender, than small plants. Reproduction was poorly correlated with environmental resource availability, measured as canopy openness, soil moisture, pH, and soil phosphorus, ammonium and nitrate. Phenotypic selection analysis on seed production suggests an optimal gender of 50-60% female, yet plasticity to be less female than this optimum, and in particular to express only male function, has been maintained. In a factorial experiment in the lab, high light or high nitrogen caused plants to produce more flowers and to be proportionally more female, and larger in weight, than low light or nitrogen. The effects of light and nitrogen on reproduction, plant size, and leaf greenness suggest an energy based determination of gender. Gender may be mostly influenced by plant size, but sometimes also opportunistically by environment. Inoculation with mycorrhizas caused plants to be less female in gender, and smaller in weight, than plants that were not inoculated. This suggests a net cost of mycorrhizas under experimental conditions, and supports the emerging view of the mycorrhizal symbiosis as not necessarily mutualistic under all circumstances.
Protandry; Sex Ratio; Tropical; Sex Allocation; Temporal Dioecy; Size Dependent Sex Determination; Pollination By Deceit; Environmental Sex Determination
Cozza, John, "Sex Expression in a Rainforest Understory Herb, Begonia urophylla" (2008). Open Access Dissertations. 186.