Publication Date
2018-09-28
Availability
Open access
Embargo Period
2018-09-28
Degree Type
Dissertation
Degree Name
Doctor of Philosophy (PHD)
Department
Biochemistry and Molecular Biology (Medicine)
Date of Defense
2018-08-24
First Committee Member
Chaitanya Jain
Second Committee Member
Antoni Barrientos
Third Committee Member
Murray P. Deutscher
Fourth Committee Member
Carlos Moraes
Fifth Committee Member
Daniel Bogenhagen
Abstract
Mammalian mitochondrial ribosomes (mitoribosomes) synthesize thirteen proteins, essential components of the oxidative phosphorylation system. They are linked to mitochondrial disorders, often involving cardiomyopathy. Mitoribosome biogenesis is assisted by multiple cofactors whose specific functions remain largely uncharacterized. Here, we examined the role of human MTG1, a conserved ribosome assembly GTPase. MTG1-silencing in human cardiomyocytes and developing zebrafish revealed early cardiovascular lesions. A combination of gene-editing and biochemical approaches using HEK293T cells demonstrated that MTG1 binds to the large subunit (mtLSU) 16S rRNA to facilitate incorporation of late assembly proteins. Furthermore, MTG1 interacts with mtLSU uL19 protein and mtSSU mS27, a putative GTP-exchange factor (GEF), to enable MTG1 release and the formation of the mB6 intersubunit bridge. In this way, MTG1 establishes a quality control checkpoint in mitoribosome assembly. In conclusion, MTG1 controls mitochondrial translation by coupling mtLSU assembly with intersubunit bridge formation using the intrinsic GEF activity acquired by the mtSSU through mS27, a unique occurrence in translational systems.
Keywords
Mitochondria; mitoribosome; mitocondrial translation; mitochondrial diseases
Recommended Citation
Kim, Hyun-Jung, "Role of GTPase MTG1 in Mitochondrial Translation and heart Physiology" (2018). Open Access Dissertations. 2189.
https://scholarlyrepository.miami.edu/oa_dissertations/2189