Doctor of Philosophy (PHD)
Marine Biology and Ecology (Marine)
Date of Defense
First Committee Member
Michael C. Schmale
Second Committee Member
Margie F. Oleksiak
Third Committee Member
M. Danielle McDonald
Fourth Committee Member
Fifth Committee Member
Damselfish neurofibromatosis (DNF) is a type of cancer affecting Stegastes partitus (Bicolor damselfish) on Florida reefs. The agent which causes the disease, termed the damselfish virus-like agent (DVLA), is the only known agent to replicate within mitochondria of animal cells. Determining the effects DVLA has on mitochondrial function and DNA levels are of importance to further understand DNF, as well as which mechanisms of mitochondrial function are directly affected by DVLA which may cause cancer. Experiments analyzed DNA levels of both DVLA and mitochondria (mtDNA), as well as mitochondrial-mediated metabolism on healthy and tumor tissues. Cell lines were also analyzed for DNA levels and metabolism, as well as mitochondrial mass and mitochondrial membrane potential. DNA results for both tissues and cell lines suggested DVLA DNA competes with mtDNA for replicative factors and decreases mtDNA replication through some unknown mechanism. Metabolic results of nodular tumors suggested mitochondrial-mediated metabolism is significantly decreased for several respiratory states/complexes in comparison to healthy skin, while cell lines metabolic results were inconclusive due to the cellular environment created by the supplemented growth media used in vitro. Overall, the presence of DVLA within tissues may initiate a cascade of events that initially decreases mtDNA replication, leading to decreases in mtRNA synthesis, which may trigger a cascade of events that upregulates/downregulates cell signaling pathways. Changes related to cell signaling pathways may include deregulated metabolism, sustaining proliferative signaling and resisting cell death, which are all hallmarks of cancer.
Mitochondria; Cancer; Damselfish; Neurofibromatosis; Virus; Metabolism
Ovares, Merly Laura, "Infection of Mitochondria in Damselfish Neurofibromatosis: Impacts on Mitochondrial Function and DNA Levels" (2019). Open Access Dissertations. 2424.
Available for download on Saturday, December 11, 2021