Publication Date
2014-12-05
Availability
Open access
Embargo Period
2014-12-05
Degree Name
Master of Science (MS)
Department
Marine Affairs and Policy (Marine)
Date of Defense
2014-11-03
First Committee Member
Daniel D. Benetti
Second Committee Member
Maria L. Estevanez
Third Committee Member
Federico Rotman
Abstract
The rotifer (Brachionus plicatilis) is an essential feed organism for successful marine larviculture. Cost effective feeding regimes and the control of harmful bacteria are critical components of rotifer production. Supplementation of commercial diets with yeast can reduce costs, but its effects on bacteria such as Vibrio spp. are not well documented. Two experiments were conducted to identify a practical culture diet. The first experiment evaluates different commercial diets on rotifer growth and total Vibrio. The second experiment determines a practical level of yeast substitution, with regard to rotifer growth, culture Vibrio loads, and cost. The diets evaluated in the first trial include INVE S.parkle, Reed RotiGrow Nannochloropsis, Reed Tetraselmis, and Aquafauna Bio-marine Algamac Protein Plus. The second trial substituted yeast for 50%, 75%, and 85% of the top performing commercial diet from the first trial. Total rotifer growth (%) and Vibrio (CFU rotifer-1) were measure in both trials, and results were evaluated with one-way ANOVAs and Tukey post-hoc tests. Rotifer growth was highest for INVE S.parkle and Reed RotiGrow Nannochloropsis. Reed Tetraselmis contained the lowest Vibrio (CFU rotifer-1) on day 2 and was significantly different than all other treatments. In the second experiment, no significant differences were found in growth between levels of yeast substitution for S.parkle. There was, however, a significantly greater concentration of Vibrio within the 75% substitution group on day 3.
Keywords
Rotifer; diet; yeast
Recommended Citation
Lind, Ryan D., "Effects of Selected Commercial Diets and Yeast Substitution on the Growth and Associated Microbiota of Rotifer (Brachionus plicatilis)" (2014). Open Access Theses. 527.
https://scholarlyrepository.miami.edu/oa_theses/527