Document Type

Internship Report

Publication Date

Spring 2014


Leukocytes, or white blood cells, include neutrophils, eosinophils, basophils, lymphocytes, and monocytes, and each has a distinct role in the immune system of animals. The manual leukocyte differential count is a method of evaluating leukocyte types, ratios, and morphology to monitor animal health and diagnose various diseases and conditions. Automated machinery in the form of automated cell counters and automated image analysis systems have proven useful in evaluating leukocytes more effectively and efficiently than the manual differential. The Cellavision DM96 is one of the newest automated image analysis systems available to pathology labs, while the Hemavet 950FS Multispecies Hematology Analyzer is a trusted veterinary hematological system. The goal of this study was to compare leukocyte differential counts of Atlantic bottlenose dolphins (Tursiops truncatus) performed by the Cellavision DM96, Hemavet 950, and the manual method. Samples from 8 animals were used to compare results of the three methods. Results indicated that the DM96 corresponds well with the manual method when analyzing neutrophils and eosinophils, while the Hemavet 950 outperforms the DM96 when measuring lymphocytes, monocytes, and basophils. The DM96 exhibited lower total bias than the Hemavet. Future studies should further explore the capabilities of the DM96 with dolphin blood samples by utilizing a larger and more diverse sample of animals.


Department: MBF

MPS Track: MMS

Location: Dolphins Plus (Key Largo, FL )

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